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b2 addgene cat 58779  (Addgene inc)


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    Structured Review

    Addgene inc b2 addgene cat 58779
    B2 Addgene Cat 58779, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b2+addgene+no+58779/pX330S-3+(Plasmid+%2358779)/pmc09217744__41586_2022_4803_MOESM1_ESM-252-40-41
    Average 93 stars, based on 12 article reviews
    b2 addgene cat 58779 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Multiplexing:

    Article Title: LADL: light-activated dynamic looping for endogenous gene expression control.
    Article Snippet: The Addgene no. 58768 plasmid was digested with SnaBI and EcoRI to excise the Cas9 open reading frame and ligated with the annealed and phosphorylated dsDNA oligo (MRP173, MRP174; see Supplementary Table 1) to get the ampicillin-resistant S12.1 gRNA multiplex backbone plasmid (Supplementary Fig. 2d). .. For step 1 of multiplexing, sgRNAs were cloned into one of the following plasmids: S12.1 (ampicillin resistant) (Supplementary Fig. 2e), B1 (Addgene no. 58778)20 (Supplementary Fig. 2f), B2 (Addgene no. 58779)20 (Supplementary Fig. 2g) or B3 (Addgene no. 58780)20 (Supplementary Fig. 2h). ..

    Clone Assay:

    Article Title: LADL: light-activated dynamic looping for endogenous gene expression control.
    Article Snippet: The Addgene no. 58768 plasmid was digested with SnaBI and EcoRI to excise the Cas9 open reading frame and ligated with the annealed and phosphorylated dsDNA oligo (MRP173, MRP174; see Supplementary Table 1) to get the ampicillin-resistant S12.1 gRNA multiplex backbone plasmid (Supplementary Fig. 2d). .. For step 1 of multiplexing, sgRNAs were cloned into one of the following plasmids: S12.1 (ampicillin resistant) (Supplementary Fig. 2e), B1 (Addgene no. 58778)20 (Supplementary Fig. 2f), B2 (Addgene no. 58779)20 (Supplementary Fig. 2g) or B3 (Addgene no. 58780)20 (Supplementary Fig. 2h). ..

    Plasmid Preparation:

    Article Title: LADL: light-activated dynamic looping for endogenous gene expression control.
    Article Snippet: Initially, CRY2 was cloned into S12.1 using the EcoRI and SnaBI sites, and then gRNA 115 was cloned into S12.1. gRNA 117 was cloned into B1 (Addgene no. 58778)20. .. We multiplexed gRNA 115 plasmid and gRNA 117 plasmid as well as B2 (Addgene no. 58779)20 and B3 (Addgene no. 58780)20 together to give the ‘onesided guide control’ plasmid (Supplementary Fig. 2m and Supplementary Table 6). ..

    Control:

    Article Title: LADL: light-activated dynamic looping for endogenous gene expression control.
    Article Snippet: Initially, CRY2 was cloned into S12.1 using the EcoRI and SnaBI sites, and then gRNA 115 was cloned into S12.1. gRNA 117 was cloned into B1 (Addgene no. 58778)20. .. We multiplexed gRNA 115 plasmid and gRNA 117 plasmid as well as B2 (Addgene no. 58779)20 and B3 (Addgene no. 58780)20 together to give the ‘onesided guide control’ plasmid (Supplementary Fig. 2m and Supplementary Table 6). ..



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